Sample Submission
Samples can be delivered to the LRGC between 8:30 am and 4:30 pm, Monday to Friday.
Alternatively, samples can be shipped via courier on dry ice.
Please complete the appropriate form to accompany your samples.
Please use this shipping address.
RNA-Seq
- 100 -1,000 ng of total RNA in at most 11 uL of DNase/RNase free water
- A260/280 > 1.8
- A260/230 > 1.8
- Bioanalyzer electropherogram showing no RNA degradation
- Illumina Recommendations
DNA-seq
- 10 uL at 1 ng/uL DNA in DNase/RNase free water
Full Plasmid Sequencing
- 10 uL of DNA at 30 ng/uL in water (by Qubit)
- A260/280 > 1.8 (by Nanodrop)
- A260/230 > 1.8 (by Nanodrop)
- Details: Flow Genomics
Sanger Sequencing
miRNA-seq
- 500 ng of total RNA in at most 5 uL of DNase/RNase free water
ChIP-seq
- 5 - 1,000 ng of fragmented DNA in at most 50 uL of DNase/RNase free water
Single Cell Gene Expression
- 100 ul at 500 - 1,200 cells/uL in buffer
Spatial Transcriptomics
- DV200% > 30%
Agilent Bioanalyzer
- RNA 6000 Nano: 3 uL of total RNA at 50 - 500 ng/uL in DNase/RNase free water
- RNA Pico: 3 uL of total RNA at 50 - 5,000 pg/uL in DNase/RNase free water
- DNA High Sensitivity: 3 uL of DNA at 100 - 5,000 pg/uL in DNase/RNase free water
