Molecular Pathology :: Robarts Research Institute

Molecular Pathology
Robarts Research Institute
100 Perth Drive, Rm. 4242
London, Ontario,
Canada N6A 5K8
(519) 663-5777 Ext. 24407
molpath@robarts.ca

The Molecular Pathology Core Facility entails a full spectrum of state-of-art histopathology equipment and expertise. The facility enables investigators to study fresh, frozen, or paraffin-processed tissues and analyze sections using various histological stains and molecular probes. In addition, micro-isolation of discrete cells within tissue sections can be accomplished using laser capture microscopy.

The facility is flexibly structured so that the work can be done by experienced technical staff or be trained to use the equipment themselves. Pricing has been set up accordingly.

The Molecular Pathology core lab is equipped with a Leica ASP300 fully enclosed paraffin wax tissue processor. The ASP300 has a capacity to process up to 300 samples at a time and can be done over night. More

The Core Facility has two cryostats, three micotomes, and two vibratomes available for tissue sectioning. Microm HM335E Microtome Leica CM350 Cryostat Leica VT1000S Vibratome. More

The core facility operates a Leica Autostainer XL for H&E stains and slide preperation for special stains. The stainer is capable of running multiple programs, concurrently, which ensures a quick turn around. More

The Molecular Pathology Facility offers many staining techniques including Immunohistochemistry ( IHC) or Immunofluorescence (IF), the process of localizing proteins in cells of tissue sections using the principle of antibodies binding specifically to antigens. Localization of these proteins can be customized specifically to the researchers needs. More

Laser Capture Microdissection is a method for procuring pure cells from specific microscopic regions of tissue sections. Laser Capture Microdissection allows researchers to obtain homogeneous cell types and multi-cellular structures isolated from whole tissue or cytology samples. The captured cells can then be used in a wide range of downstream assays, such as gene expression analysis, genomic profiling using microarrays, or for proteomic analysis. More Info