Laser Capture Microscope

The Robart's Molecular Pathology core lab's Veritas Laser Capture microdissection (LCM) instrument combines a solid-state UV cutting laser with an IR laser allowing precise control and capture of the intended area of interest. The benefit of the dual laser systems allows greater flexibility and speed while providing a gentler dissection method that reduces heat damage to the cells without affecting the chemistry or morphology.

The process for capturing the cells in the area of interest is done using a thin, thermoplastic film (CapSure LCM) that is places over the target and gently softened with laser pulses. The CAP adheres to the specific region and is lifted away from the slide where the thermoplastic solidifies with the captured cells. The CAP can then be centrifuged with buffer to extract the RNA, DNA or proteins from the captured cells. The LCM is available to use through the Robarts Pathology facility.

Training on the instrument can be arranged by contacting Kelly or Darcy at 519-663-5777 ext. 24407 or via email at molpath@robarts.ca For more information on how laser capture can benefit your research or the capabilities of the Veritas LCM please see the following resources:

Molecular Pathology Protocol

LCM Research Papers

ArcturusXT Laser Capture Microdissection System

Laser Cature Information
Sample Protocol
Journal Nature: Protocol