London Regional Genomics Centre

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The London Regional Genomics Centre is a Robarts-led initiative by Dr. Robert Hegele and has been established through generous support from the Robarts Research Institute, the Schulich School of Medicine & Dentistry, London Health Sciences Centre, Child Health Research Institute and The London Regional Cancer Program. The facility provides state-of-the-art services on a fee-for-service/cost recovery basis and offers Affymetrix microarrays, Next-Generation Sequencing on the Illumina MiSeq and Ion Torrent PGM platforms, and data analysis software.

Services provided by the facility include qualitative assessment of RNA via Agilent 2100 Bioanalyzer and Consultation on experimental design.

Next Generation Sequencing:
- Ion Torrent Personal Genome Machine
- Illumina MiSeq

Affymetrix GeneChip system:
- Gene expression, exon, ChIP and tiling arrays
- SNP genotyping and copy number
- GeneChip scanner 3000 7G with autoloader

Data Analysis:
- Partek, CLCbio Genomics Workbench and Agilent GT
- Pathway analysis using Ingenuity Pathway Analysis and Partek Pathway

For a downloadable PDF, click here.

Facility Manager: David Carter, microarray@robarts.ca, 519-931-5246
Location:              Robarts Research Institute, Room 4294
Active Users:       260+ PIs in Canada
Fee Structure:      For full fee structure, see: http://www.lrgc.ca/?page=fees

www.lrgc.ca



DNA Sequencing

Under the scientific direction of Dr. Robert Hegele, the DNA Sequencing Facility at Robarts provides high quality sample analysis - generally within 48 hours of submission - using state-of-the-art equipment, including an ABI GeneAmp 9600 Thermocycler, three ABI GeneAmp 9700 Thermocyclers and one Applied Biosystems 3730 Analyzer.

Scientific Director:   Dr. Robert Hegele
Location:                 Robarts Research Institute, Room 4212
Active Users:          250+ PIs in Canada
Fee Structure:        Standard Sequencing reaction - $6
                                Sequencing reaction with dGTP added - $7
                                Fragment analysis and SNapShot - $6
                                Large DNA (Genomic, BAC’s, Cosmids, Fosmid) - $20
                                Commercial Sequencing - $12
                                Real-Time PCR - $40 per run
Discounts are available for large volume projects; please contact the facility to discuss      



Sample Preparation    Primers    Data Interpretation    Request Form     Facility Description